Advanced Light Imaging and Spectroscopy (ALIS) facility

The Advanced Light Imaging and Spectroscopy (ALIS) facility provides cutting-edge optical imaging technology that is beyond the capabilities of commercial instruments.

The 980-square-foot facility represents a significant investment in the sciences at Duke, and complements the capabilities of the Light Microscopy Core Facility.

The first instruments in the facility include a lattice light-sheet microscope, a deep-tissue serial sectioning microscope, and a multimodal pump-probe microscope. ALIS will offer the possibility of adapting these microscopes and co-developing novel microscopy approaches with PIs on campus.

For more information, visit the ALIS website:
Collage featuring a microscope, and microscopy images in red, green, and pink

ALIS currently provides access to :

Lattice Light Sheet Microscope

•High speed volumetric imaging for live samples, e.g., cells, organelles, Drosophila and Zebrafish embryos

•High spatial resolution: up to 230 x 230 x 370 nm (even better in SIM mode)

•Fast acquisition: typically (100 µm)3 3D stack in less than a second

•Large imaging depth (up to ~75 µm) in weakly scattering specimens

•Significantly reduced photobleachingand phototoxicityfor long observation times


Pump-Probe Microscope (experimental)

•Transient absorption contrast

•Combined with multi-photon fluorescence


Deep-Tissue Sectioning Microscope

•Multi-photon fluorescence contrast

•Automated sectioning of large embedded samples (~cm3), e.g., mouse brains


ALIS also offers:

•Adapting microscopes to your application

•Co-developing novel microscopes

•Image analysis consultation/service