The Advanced Light Imaging and Spectroscopy (ALIS) facility provides cutting-edge optical imaging technology that is beyond the capabilities of commercial instruments.
The 980-square-foot facility represents a significant investment in the sciences at Duke, and complements the capabilities of the Light Microscopy Core Facility.
The first instruments in the facility include a lattice light-sheet microscope, a deep-tissue serial sectioning microscope, and a multimodal pump-probe microscope. ALIS will offer the possibility of adapting these microscopes and co-developing novel microscopy approaches with PIs on campus.
For more information, visit the ALIS website: https://alis.duke.edu
ALIS currently provides access to :
Lattice Light Sheet Microscope
•High speed volumetric imaging for live samples, e.g., cells, organelles, Drosophila and Zebrafish embryos
•High spatial resolution: up to 230 x 230 x 370 nm (even better in SIM mode)
•Fast acquisition: typically (100 µm)3 3D stack in less than a second
•Large imaging depth (up to ~75 µm) in weakly scattering specimens
•Significantly reduced photobleachingand phototoxicityfor long observation times
Pump-Probe Microscope (experimental)
•Transient absorption contrast
•Combined with multi-photon fluorescence
Deep-Tissue Sectioning Microscope
•Multi-photon fluorescence contrast
•Automated sectioning of large embedded samples (~cm3), e.g., mouse brains
ALIS also offers:
•Adapting microscopes to your application
•Co-developing novel microscopes
•Image analysis consultation/service